Chow Lab @ Penn Bioengineering

Resources


Summary: We freely share our tools with the academic and non-profit community to maximize their proliferative benefit. We welcome any feedback that you may have.

Plasmids

The plasmid collection is available at Addgene.

luxR plasmids for exploring modular gene regulation (as described in Magaraci+, ACS Synthetic Biology, 2016).

melanopsin plasmids for optogenetic activation of Gq signaling (as described in Hannanta-Anan and Chow, Cell Systems, 2016).

opto-RGS2 plasmids for optogenetic suppression of Gq signaling (as described in Hannanta-Anan and Chow, ACS Synthetic Biology, 2018).

RGS-LOV (BcLOV4) plasmids for single-component optogenetic membrane recruitment (as described in Glantz et al., PNAS, 2018).

dFP plasmids (as described in Sheehan et al., Biochemistry, 2018).

Hardware

24-well plate illuminator for optogenetic induction (as described in Hannanta-anan and Chow, Cell Systems, 2016): CAD files are available at Penn Scholarly Commons, where updates will be posted.

Low-cost transilluminator for automated and web-enabled gel/plate documentation (as described in Magaraci+, ACS Synthetic Biology, 2016): CAD files and assembly instructions are available in the manuscript supplementary material online. The ISO file for RaspberryPi installation and initialization are available at Penn Scholarly Commons, where updates will be posted.

Open-source plate reader (OSP) (as described in Szymula+, Biochemistry, 2019): CAD files and assembly instructions are available in [supplementary material]. All files, including the ISO file for RaspberryPi installation and initialization are available at Github and Penn Scholarly Commons, where updates will be posted. CAD files are also available at MetaFluidics.

Datasets and Software

LOV domain annotations for ontological function and conserved domain topology (as described in Glantz+, PNAS, 2016): The entire dataset is available as an Excel spreadsheet in the supplementary material online.

Synthetic cell segmentation for identifying interfaces and bulk compartments of emulsion droplets (as described in Glantz+, Meth. Enzym., 2019): The code is available in the supplementary material online.